Blast dastur yordamida nukleotidlar ketma ketligini taqqoslash - Информатика и ИТ - Презентации

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Blast dastur yordamida nukleotidlar ketma ketligini taqqoslash

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Blast dastur yordamida nukleotidlar ketma ketligini taqqoslash The user can demonstrate on a projector or computer, or print the presentation and make it film

REJA 01 0302 Blast dasturi òzi nima? Blast dasturi qanday ishlaydi Blast nukleotidlar ketma ketligi dasturi

01 Blast dasturi òzi nima?

Title BLAST (Basic Local Alignment Search Tool – asosiylokal tekislanishni izlash vositasi) bu kompyuter dasturlaridan biri bo’lib, u oqsil yoki nuklein kislotalar gomologlarini mavjud bazadan izlab topishga xizmat qiladi. Sodda qilib tushuntirilganda sizda oqsil yoki nuklein kislotalarning ma’lumbir ketma-ketligi bor.

Lekin siz bu nuklein kislota qaysi turga tegishli ekanligini bilmaysiz. BLAST sizga mana shu qo’lingizdagi nukleotidlar ketma- ketligi qaysi turga tegishli ekanligini aniqlashga yordam beradi. Albattau bazada mavjud bo’lgan millionlab namunalarga solishtirish asosida sizga kerakli natijani chiqarib beradi.

BLAST dasturini 1990- yilda AQSHlik bir guruh tadqiqotchilar ishlab chiqqanlar.

Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmBu tasvirda DNK ikkala zanjirining ma’lum bir fragmenti va undagi nukleotidlarning spektrlari tasvirlangan. DNK ning ushu holatini ko’rish uchun “ContigExpress Project” bioinformatsion dasturidan foydalaniladi.

02 Blast dasturi qanday ishlaydi

baliq namunasining to’qimasidan mitoxondriya DNK ni ajratib olib (bu alohida mavzu), mitoxondriyadagi COI genini PZR mashinasida amplifikatsiya qilamiz COI geni o’rtacha 700 ta nukleotiddan iborat bo’ladi. Biz DNK zanjirlarining holatini tekshirib ulardan umumiy holatda quyidagi nukleotidlar ketma-ketligini qo’lgan kiritamiz.

02 0501 0403Title text additionThe user can demonstrate on a projector or computer, or print the presentation and make it film Title text addition Title text addition Title text addition Title text additionThe user can demonstrate on a projector or computer, or print the presentation and make it film The user can demonstrate on a projector or computer, or print the presentation and make it film The user can demonstrate on a projector or computer, or print the presentation and make it film The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition

VSMana bizda 723 ta nukleotidlardan tashkil topgan DNK ning bitta zanjiri fragmentining nukleotidlari turibdi. Ana endi uni BLAST yordamida kimga tegishli ekanligini topib olamiz.

03 Blast nukleotidlar ketma ketligi dasturi

BLAST ga qanday kiriladi? Buning uchun kompyuterda internetni yoqib AQSHning Biotexnologik ma’lumotlar milliy markazi (NCBI) saytiga kiramiz. U yerdan BLAST bo’limi tanlaymiz. Bizda quyidagi oyna ochiladi.

Biz nukleotidlar ketma- ketligini tekshirmoqchi bo’lganimiz uchun chap tomonda turgan Nucleotide BLAST tugmasini bosamiz. Kompyuter ekranida quyidagi oyna ochiladi.1A1222040A05 13030E1512 25 0E 1A1B1C1D01 30 0D

Title text addition Nukleotidlarimizni birinchi yuqoridagi qizil ko’rsatgichda ko’rsatilgan joyga ko’chirib olib qo’yamiz (Ctrl+C va Ctrl+V). Shundan so’ng ikkinchi ko’rsatkich ko’rsatayotgan joy belgilanganligini tekshiramiz. Bu nuqta siz yuklagan nukleotidlarni qaysi ma’lumotlar ba’zasi bilan solishtirish kerakligini aniqlashtirish uchun kerak.

1 2 3 4Oynaning yuqori qismida BLAST amalga oshirilgan operatsiya haqida qisqacha ma’lumot va bizning nukleotidlar solishtirilishidan qo’lgan kiritilgan dastlabki 100 ta eng o’xshash natijalarning diagramma holatidagi tasviri ko’rsatiladi.

Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it film Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition The user can demonstrate on a projector or computer, or print the presentation and make it film Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition

Step 01 Step 02 Step 03 Step 04 Step 05 Step 06Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it film Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it film Title text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmTitle text addition The user can demonstrate on a projector or computer, or print the presentation and make it filmOynaning yana bir pastrog’iga tushsak, mana shu har bir natija ko’rsatkichi bilan bizdagi ko’rsatkich qanday solishtirilgani ko’rsatiladi.

Demak xulosa sifatida biz tekshirayotgan organizm Pseudorasbora parva ekanligini bilib oldik. Ushbu baliqni to’g’ri aniqlaganimizni fishbase.org sayti orqali tekshirib ko’ramiz. U yerdagi bizga quyidagi baliqning ma’lumotlari ko’rsatilmoqda.

Etiboringiz uchun raxmat The user can demonstrate on a projector or computer, or print the presentation and make it film

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